Transcriptome Analyses in BV2 Microglial Cells Following Treatment With Amino-Terminal Fragments of Apolipoprotein E.

ABSTRACT: Despite the fact that harboring the apolipoprotein E4 (APOE4) allele represents the single greatest risk factor for late-onset Alzheimer's disease (AD), the exact mechanism by which ApoE4 contributes to disease progression remains unknown. Recently, we demonstrated that a 151 amino-terminal fragment of ApoE4 (nApoE4_1-151) localizes within the nucleus of microglia in the human AD brain and traffics to the nucleus causing toxicity in BV2 microglia cells. In the present study, we examined in detail what genes may be affected following treatment by nApoE4_1-151. Transcriptome analyses in BV2 microglial cells following sublethal treatment with nApoE4_1-151 revealed the upregulation of almost 4,000 genes, with 20 of these genes upregulated 182- to 715-fold c

SUBMITTER: Pollock TB 

PROVIDER: S-EPMC7456952 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature