SARS-CoV-2 genomic and quasispecies analyses in cancer patients reveal relaxed intrahost virus evolution.

ABSTRACT: Numerous factors have been identified to influence susceptibility to SARS-CoV-2 infection and disease severity. Cancer patients are more prone to clinically evolve to more severe COVID-19 conditions, but the determinants of such a more severe outcome remain largely unknown. We have determined the full-length SARS-CoV-2 genomic sequences of cancer patients and healthcare workers (HCW; non-cancer controls) by deep sequencing and investigated the within-host viral quasispecies of each infection, quantifying intrahost genetic diversity. Naso- and oropharyngeal SARS-CoV-2 + swabs from 57 cancer patients and 14 healthcare workers (HCW) from the Brazilian Cancer Institute were collected in April-May 2020. Complete genome amplification using ARTIC network V3 multiplex primers was performed followe

SUBMITTER: Siqueira JD 

PROVIDER: S-EPMC7457605 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature