Dataset Information

GCNT4 is Associated with Prognosis and Suppress Cell Proliferation in Gastric Cancer.

ABSTRACT:

Background

GCNT4 is a member of the glucosaminyl (N-acetyl) transferases family that has been implicated in multiple human malignancies. However, the role of GCNT4 in gastric cancer (GC) is unknown. In this present study, we aimed to explore the role and clinicopathological correlation of GCNT4 in GC.

Materials and methods

We first evaluated the dysregulation of GCNT4 in The Cancer Genome Atlas (TCGA) and then we performed RT-qPCR and immunohistochemistry to validate the results in a cohort of in-house patients. The clinicopathological correlation and function of GCNT4 in GC were also analysed.

Results

GCNT4 was found to be significantly downregulated in GC. In addition, GCNT4 expression correlated with tumour depth, nervous invasion and pathological tumor-node-metastasis (pTNM) stage. Moreover, lower GCNT4 levels conferred poor overall survival (OS) and disease-free survival (DFS) to GC patients. Multivariate Cox regression analysis revealed that GCNT4 protein expression is an independent prognostic factor for OS in patients with GC. Further functional experimental results revealed that overexpression of GCNT4 appears to halt GC cell proliferation and the cell cycle.

Conclusion

Altogether, these findings indicated that GCNT4 regulates the GC cell cycle and have important implications for the selection of therapeutic targets to prevent tumour proliferation.

SUBMITTER: Sun H

PROVIDER: S-EPMC7457769 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

GCNT4 is Associated with Prognosis and Suppress Cell Proliferation in Gastric Cancer.

Sun Hui H Chang Jinjia J Ye Min M Weng Weiwei W Zhang Meng M Ni Shujuan S Tan Cong C Huang Dan D Wang Lei L Du Xiang X Xu Mi-Die MD Sheng Weiqi W

OncoTargets and therapy 20200825

<h4>Background</h4>GCNT4 is a member of the glucosaminyl (N-acetyl) transferases family that has been implicated in multiple human malignancies. However, the role of GCNT4 in gastric cancer (GC) is unknown. In this present study, we aimed to explore the role and clinicopathological correlation of GCNT4 in GC.<h4>Materials and methods</h4>We first evaluated the dysregulation of GCNT4 in The Cancer Genome Atlas (TCGA) and then we performed RT-qPCR and immunohistochemistry to validate the results i ...[more]

PMID: 32922038

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Dataset Information

GCNT4 is Associated with Prognosis and Suppress Cell Proliferation in Gastric Cancer.

Background

Materials and methods

Results

Conclusion

Publications

GCNT4 is Associated with Prognosis and Suppress Cell Proliferation in Gastric Cancer.

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