Temporal Detection and Phylogenetic Assessment of SARS-CoV-2 in Municipal Wastewater.
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ABSTRACT:
SUBMITTER: Nemudryi A
PROVIDER: S-EPMC7457911 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Temporal Detection and Phylogenetic Assessment of SARS-CoV-2 in Municipal Wastewater.
Nemudryi Artem A Nemudraia Anna A Wiegand Tanner T Surya Kevin K Buyukyoruk Murat M Cicha Calvin C Vanderwood Karl K KK Wilkinson Royce R Wiedenheft Blake B
Cell reports. Medicine 20200831 6
SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here, we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results. In addition, we determine a nearly complete (98.5%) SARS-CoV-2 genome sequence from wastewater and use phylogeneti ...[more]
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