Comparison of serum exosome isolation methods on co-precipitated free microRNAs.

ABSTRACT:

Background

Exosomes are nano-sized extracellular vesicles containing different biomolecules such as proteins and microRNAs (miRNAs) that mediate intercellular communication. Recently, numerous studies have reported the important functions of exosomal miRNAs in disease development and the potential clinical application as diagnostic biomarkers. Up to now, the most commonly used methods to extract exosomes are ultracentrifugation (UC) and precipitation-based commercial kit (e.g., ExoQuick). Generally, both UC and ExoQuick method could co-isolate contaminating proteins along with exosomes, with the UC method yielding even purer exosomes than ExoQuick. However, the comparison of these two methods on co-precipitated free miRNAs is still unknown.

Methods

In this study, we isolated

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC7457927 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature