Gene co-expression network analysis to identify critical modules and candidate genes of drought-resistance in wheat.

ABSTRACT:

Aim

To establish a gene co-expression network for identifying principal modules and hub genes that are associated with drought resistance mechanisms, analyzing their mechanisms, and exploring candidate genes.

Methods and findings

42 data sets including PRJNA380841 and PRJNA369686 were used to construct the co-expression network through weighted gene co-expression network analysis (WGCNA). A total of 1,896,897,901 (284.30 Gb) clean reads and 35,021 differentially expressed genes (DEGs) were obtained from 42 samples. Functional enrichment analysis indicated that photosynthesis, DNA replication, glycolysis/gluconeogenesis, starch and sucrose metabolism, arginine and proline metabolism, and cell cycle were significantly influenced by drought stress. Furthermore, the DEGs with si

SUBMITTER: Lv L 

PROVIDER: S-EPMC7458298 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature