Identification of a neo-epitope dominating endogenous CD8 T cell responses to MC-38 colorectal cancer.

ABSTRACT: The murine MC-38 colorectal cancer model is a commonly used model for cancer with high mutational burden, which is sensitive for immune checkpoint immunotherapy. We set out to analyze endogenous CD8⁺ T cell responses to MC-38 neo-antigens in tumor-bearing mice and after anti-PD-L1 checkpoint therapy. Through combination of whole-exome sequencing analysis with mass spectrometry of MHC class I eluted peptides we could identify eight candidate epitopes. Of these, a neo-epitope encoded by a point-mutation in the sequence of the ribosomal protein L18 (Rpl18) strongly dominated the CD8⁺ T cell response to our MC-38 cell-line in comparison to a previously described neo-epitope in the Adpgk protein. Therapeutic vaccination with synthetic peptides induced CD8⁺ T cel

SUBMITTER: Hos BJ 

PROVIDER: S-EPMC7458608 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature