Entropy of a bacterial stress response is a generalizable predictor for fitness and antibiotic sensitivity.

ABSTRACT: Current approaches explore bacterial genes that change transcriptionally upon stress exposure as diagnostics to predict antibiotic sensitivity. However, transcriptional changes are often specific to a species or antibiotic, limiting implementation to known settings only. While a generalizable approach, predicting bacterial fitness independent of strain, species or type of stress, would eliminate such limitations, it is unclear whether a stress-response can be universally captured. By generating a multi-stress and species RNA-Seq and experimental evolution dataset, we highlight the strengths and limitations of existing gene-panel based methods. Subsequently, we build a generalizable method around the observation that global transcriptional disorder seems to be a common, low-fitness, stress

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC7458919 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature