In silico characterization of synthetic promoters designed from mirabilis mosaic virus and rice tungro bacilliform virus.

ABSTRACT: CaMV35S is the most extensively used promoter for ectopic gene expression in plant system. However, multiple use of this promoter possesses several limitation i.e. homologous based gene silencing and differential suitability in monocot and dicot plants. The strength of a promoter is defined by the presence of cis-acting elements and trans acting nucleic binding factors, thus its strength can be regulated by changing the architecture of these regulatory elements. In the present study, eight hybrid promoters were designed from two parareteroviruses, rice tungro bacilliform viruses (RTBV) and mirabilis mosaic virus (MMV). The eight hybrid promoters, along with parental promoters were characterized for the presence of functional cis-elements and transcription factor binding sites

SUBMITTER: Gupta D 

PROVIDER: S-EPMC7459046 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature