Single-cell RNA sequencing uncovers heterogenous transcriptional signatures in macrophages during efferocytosis.

ABSTRACT: Efferocytosis triggers cellular reprogramming, including the induction of mRNA transcripts which encode anti-inflammatory cytokines that promote inflammation resolution. Our current understanding of this transcriptional response is largely informed from analysis of bulk phagocyte populations; however, this precludes the resolution of heterogeneity between individual macrophages and macrophage subsets. Moreover, phagocytes may contain so called "passenger" transcripts that originate from engulfed apoptotic bodies, thus obscuring the true transcriptional reprogramming of the phagocyte. To define the transcriptional diversity during efferocytosis, we utilized single-cell mRNA sequencing after co-cultivating macrophages with apoptotic cells. Importantly, transcriptomic analyses were performed

SUBMITTER: Lantz C 

PROVIDER: S-EPMC7459098 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature