Promising xenograft animal model recapitulating the features of human pancreatic cancer.

ABSTRACT:

Background

Multiple sites of metastasis and desmoplastic reactions in the stroma are key features of human pancreatic cancer (PC). There are currently no simple and reliable animal models that can mimic these features for accurate disease modeling.

Aim

To create a new xenograft animal model that can faithfully recapitulate the features of human PC.

Methods

Interleukin 2 receptor subunit gamma (IL2RG) gene knockout Syrian hamster was created and characterized. A panel of human PC cell lines were transplanted into IL2RG knockout Syrian hamsters and severe immune-deficient mice subcutaneously or orthotopically. Tumor growth, local invasion, remote organ metastasis, histopathology, and molecular alterations of tumor cells and stroma were compared over time.<

SUBMITTER: Miao JX 

PROVIDER: S-EPMC7459204 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature