Dataset Information

All-trans retinoic acid increases ARPE-19 cell apoptosis via activation of reactive oxygen species and endoplasmic reticulum stress pathways.

ABSTRACT:

Aim

To explore the apoptosis of ARPE-19 cells after the treatment with different doses of all-trans-retinoic acid (ATRA).

Methods

ARPE-19 cells were used in the in-vitro experiment. Flow cytometry assay was employed to evaluate the level of reactive oxygen species (ROS) and apoptosis. The effects of ATRA (concentrations from 2.5 to 20 µmol/L) on the expression of endoplasmic reticulum stress (ERS) markers in vitro were evaluated by Western blot and real-time quantitative polymerase chain reaction (qRT-PCR) assays. The contribution of ROS and ERS-induced apoptosis in vitro was determined by using N-acetyl-L-cysteine (NAC) and Salubrinal, an antagonist of NAC and ERS, respectively.

Results

Flow cytometry showed that ATRA significantly increased ARPE-19 cell apoptosis and ROS levels in each group (F=86.39, P<0.001; F=116.839, P<0.001). Western blot and qRT-PCR revealed that levels of CHOP and BIP were elevated in a concentration-dependent pattern after the cells were incubated with ATRA (2.5-20 µmol/L). The upregulation of VEGF-A and CHOP induced by ATRA could be inhibited by NAC (antioxidant) and Salubrinal (ERS inhibitor) in vitro.

Conclusion

ATRA induces the apoptosis of ARPE-19 cells via activated ROS and ERS signaling pathways.

SUBMITTER: Wu J

PROVIDER: S-EPMC7459219 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

All-trans retinoic acid increases ARPE-19 cell apoptosis <i>via</i> activation of reactive oxygen species and endoplasmic reticulum stress pathways.

Wu Juan J Gao Zhen-Ya ZY Cui Dong-Mei DM Li Hong-Hui HH Zeng Jun-Wen JW

International journal of ophthalmology 20200918 9

<h4>Aim</h4>To explore the apoptosis of ARPE-19 cells after the treatment with different doses of all-trans-retinoic acid (ATRA).<h4>Methods</h4>ARPE-19 cells were used in the <i>in-vitro</i> experiment. Flow cytometry assay was employed to evaluate the level of reactive oxygen species (ROS) and apoptosis. The effects of ATRA (concentrations from 2.5 to 20 µmol/L) on the expression of endoplasmic reticulum stress (ERS) markers <i>in vitro</i> were evaluated by Western blot and real-time quantita ...[more]

PMID: 32953569

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