3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls.

ABSTRACT: Systemic arterial hypertension is a highly prevalent chronic disease associated with hypertensive cardiomyopathy. One important feature of this condition is remodelling of intramural small coronary arteries and arterioles. Here, we investigated the implications of this remodelling in the downstream vascular organization, in particular at the capillary level. We used Spontaneously Hypertensive Rats (SHR) exhibiting many features of the human hypertensive cardiomyopathy. We generated 3D high-resolution mesoscopic reconstructions of the entire network of SHR hearts combining gel-based fluorescent labelling of coronaries with a CLARITY-based tissue clearing protocol. We performed morphometric quantification of the capillary network over time to assess capillary diameter, linear density, and an

SUBMITTER: Olianti C 

PROVIDER: S-EPMC7459314 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature