CXCR4^hi effector neutrophils in sickle cell anemia: potential role for elevated circulating serotonin (5-HT) in CXCR4^hi neutrophil polarization.

ABSTRACT: Leukocyte recruitment and heterocellular aggregate formation drive the inflammatory vaso-occlusive processes associated with sickle cell anemia (SCA). We characterized neutrophils in a population of patients with SCA and investigated whether platelet-derived molecules can induce phenotypic alterations in this cell type. Imaging flow cytometry analysis demonstrated that the frequency of circulating CXCR4^hi neutrophils was significantly higher in steady-state SCA individuals than in healthy control individuals and that these cells presented increased CD11b activation and toll-like receptor-4 expression. SCA neutrophils display increased neutrophil-platelet aggregation, and CXCR4^hi neutrophils demonstrated augmented neutrophil-platelet aggregate frequency with a higher m

SUBMITTER: Garcia F 

PROVIDER: S-EPMC7459317 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature