Contact Effect of a Methylobacteriumsp. Extract on Biofilm of a Mycobacteriumchimaera Strain Isolated from a 3T Heater-Cooler System.

ABSTRACT: Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-treating a surface with a Methylobacterium sp. CECT 7180 extract to inhibit the M. chimaera ECMO biofilm as well as of the treatment after different dehydration times. Surface adherence, biofilm formation and treatment effect were evaluated by estimating colony-forming units (CFU) per square centimeter and characterizing the amount of covered surface area, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image an

SUBMITTER: Pradal I 

PROVIDER: S-EPMC7460266 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature