A Proposed Procedure for Discriminating between Nasal Secretion and Saliva by RT-qPCR.

ABSTRACT: In forensic casework, nasal secretion can be a good source of DNA. Moreover, saliva can prove useful in cases of sexual assault. However, discriminating between these body fluids is often difficult because of cross-reactivity between them on presumptive and confirmatory tests. Therefore, an RT-qPCR procedure was developed to discriminate between nasal secretion and saliva. Characteristic genes in nasal secretion and/or saliva (BPIFA1, STATH, HTN3, and PRH2) were selected as candidates. Discrimination criteria were established based on the expression levels of these markers in various body fluids. In addition, a flowchart was proposed and used to discriminate among nasal secretion, saliva, and other body fluids in various forensic samples. BPIFA1 was highl

SUBMITTER: Akutsu T 

PROVIDER: S-EPMC7460356 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature