Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications.

ABSTRACT: A functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such goals, we herein obtained primary goat HMCs via a microdissection-based method; optimized the selection of the culture medium and coating substances for better cell maintenance; and exemplified their usefulness through examining the effects of calcium and all-trans retinoic acid (ATRA) on cells using immunoblotting, flow cytometry, and other techniques. As a result, we successfully acquired primary and passaged goat HMCs with typical keratinocyte morphology. Calcium-free RPMI (Roswell Park Memor

SUBMITTER: Ma S 

PROVIDER: S-EPMC7460477 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature