Multiplex Digital MicroRNA Detection Using Cross-Inhibitory DNA Circuits.

ABSTRACT: Ubiquitous post-transcriptional regulators in eukaryotes, microRNAs are currently emerging as promising biomarkers of physiological and pathological processes. Multiplex and digital detection of microRNAs represents a major challenge toward the use of microRNA signatures in clinical settings. The classical reverse transcription polymerase chain reaction quantification approach has important limitations because of the need for thermocycling and a reverse transcription step. Simpler, isothermal alternatives have been proposed, yet none could be adapted in both a digital and multiplex format. This is either because of a lack of sensitivity that forbids single molecule detection or molecular cross-talk reactions that are responsible for nonspecific amplification. Building on an ultrasensitive

SUBMITTER: Rondelez Y 

PROVIDER: S-EPMC7460561 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature