Exposure to perfluorooctanoic acid (PFOA) decreases neutrophil migration response to injury in zebrafish embryos.

ABSTRACT:

Objective

Perfluorooctanoic acid (PFOA) is a ubiquitous environmental contaminant and a known immune suppressant in humans and experimental animal models. Studies on PFOA have focused on suppression of the adaptive immune response; however, little is known of the impact on innate immunity, especially during embryogenesis. Therefore, we utilized the zebrafish chemotaxis assay coupled with in situ hybridization for myeloperoxidase expression to determine the effects of PFOA exposure on neutrophil migration in the developing zebrafish embryo. Zebrafish embryos are a well-established in vivo model that exhibit high homology with the development of human innate immunity.

Results

Treatment of zebrafish with increasing concentrations of PFOA identified the lethal concentration in 5

SUBMITTER: Pecquet AM 

PROVIDER: S-EPMC7460781 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature