A Fungal Ascorbate Oxidase with Unexpected Laccase Activity.

ABSTRACT: Ascorbate oxidases are an enzyme group that has not been explored to a large extent. So far, mainly ascorbate oxidases from plants and only a few from fungi have been described. Although ascorbate oxidases belong to the well-studied enzyme family of multi-copper oxidases, their function is still unclear. In this study, Af_AO1, an enzyme from the fungus Aspergillus flavus, was characterized. Sequence analyses and copper content determination demonstrated Af_AO1 to belong to the multi-copper oxidase family. Biochemical characterization and 3D-modeling revealed a similarity to ascorbate oxidases, but also to laccases. Af_AO1 had a 10-fold higher affinity to ascorbic acid (K_M = 0.16 ± 0.03 mM) than to ABTS (K_M = 1.89 ± 0.12 mM). F

SUBMITTER: Braunschmid V 

PROVIDER: S-EPMC7460845 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature