Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis.

ABSTRACT:

Background

Herpes simplex virus type 1 (HSV-1) keratitis is a major cause of corneal blindness in the world, and an in-depth understanding of its pathogenesis may help improve existing diagnosis and treatment. The purpose of this study is to compare and analysis the total tear protein profile of HSV-1 epithelial keratitis patients, and to quantify the potential candidate biomarkers of HSV-1 epithelial keratitis.

Methods

We investigated the proteome in tear fluid from three HSV-1 epithelial keratitis patients and three healthy control subjects using nano-scale liquid chromatography-tandem mass spectrometry (nLC-MS/MS) analysis. Functional annotation of differentially expressed proteins was done with the Gene Ontology (GO) analysis. ELISA was done to quantify the potential can

SUBMITTER: Yang H 

PROVIDER: S-EPMC7461310 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature