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Saliva sample pooling for the detection of SARS-CoV-2.

ABSTRACT: As the battle against coronavirus disease 2019 pandemic continues, an increase in workload and medical expenses have been a concern to the health care system worldwide. Developing a measure that helps to conserve the health care resource is, therefore, highly desirable, and the pooling of the specimens for testing is one of the attractive strategies. Recently, we showed that saliva could be a potential alternative specimen for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by real-time polymerase chain reaction (RT-PCR). In the present study, we performed the pooling of saliva specimens for testing by SARS-CoV-2 RT-PCR. We showed that the saliva pool of either 5 or 10 samples, by allowing the detection of either gene in the pool at an increased cycle threshold cutoff value, further performing individual sample testing in the positive pools did not compromise the detection of SARS-CoV-2.

SUBMITTER: Pasomsub E

PROVIDER: S-EPMC7461487 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Saliva sample pooling for the detection of SARS-CoV-2.

Pasomsub Ekawat E Watcharananan Siriorn P SP Watthanachockchai Treewat T Rakmanee Kingkan K Tassaneetrithep Boonrat B Kiertiburanakul Sasisopin S Phuphuakrat Angsana A

Journal of medical virology 20200929 3

As the battle against coronavirus disease 2019 pandemic continues, an increase in workload and medical expenses have been a concern to the health care system worldwide. Developing a measure that helps to conserve the health care resource is, therefore, highly desirable, and the pooling of the specimens for testing is one of the attractive strategies. Recently, we showed that saliva could be a potential alternative specimen for the detection of severe acute respiratory syndrome coronavirus 2 (SAR ...[more]

PMID: 32841429

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