Amphiregulin Aggravates Glomerulonephritis via Recruitment and Activation of Myeloid Cells.

ABSTRACT:

Background

Recent studies have identified the EGF receptor (EGFR) ligand amphiregulin (AREG) as an important mediator of inflammatory diseases. Both pro- and anti-inflammatory functions have been described, but the role of AREG in GN remains unknown.

Methods

The nephrotoxic nephritis model of GN was studied in AREG^-/- mice after bone marrow transplantation, and in mice with myeloid cell-specific EGFR deficiency. Therapeutic utility of AREG neutralization was assessed. Furthermore, AREG's effects on renal cells and monocytes/macrophages (M/M) were analyzed. Finally, we evaluated AREG expression in human renal biopsies.

Results

Renal AREG mRNA was strongly upregulated in murine GN. Renal resident cells were the most functionally relevant source of AREG. Impor

SUBMITTER: Melderis S 

PROVIDER: S-EPMC7461677 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature