Genome based evolutionary lineage of SARS-CoV-2 towards the development of novel chimeric vaccine.

ABSTRACT: The present study aimed to predict a novel chimeric vaccine by simultaneously targeting four major structural proteins via the establishment of ancestral relationship among different strains of coronaviruses. Conserved regions from the homologous protein sets of spike glycoprotein, membrane protein, envelope protein and nucleocapsid protein were identified through multiple sequence alignment. The phylogeny analyses of whole genome stated that four proteins reflected the close ancestral relation of SARS-CoV-2 to SARS-COV-1 and bat coronavirus. Numerous immunogenic epitopes (both T cell and B cell) were generated from the common fragments which were further ranked on the basis of antigenicity, transmembrane topology, conservancy level, toxicity and allergenicity pattern and population covera

SUBMITTER: Akhand MRN 

PROVIDER: S-EPMC7462568 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature