Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii.

ABSTRACT:

Background

Acinetobacter baumannii (A. baumannii) is one of the pivotal pathogens responsible for nosocomial infections, especially in patients with low immune response, and infection with carbapenem-resistant A. baumannii has been increasing in recent years. Rapid and accurate detection of carbapenem-resistance genes in A. baumannii could be of immense help to clinical staff.

Methods

In this study, a 15-μL reaction system for recombinase polymerase amplification (RPA) was developed and tested. We collected 30 clinical isolates of A. baumannii from the Burn Institute of Southwest Hospital of Third Military Medical University (Army Medical University) for 6 months and tested antibiotic susceptibility using the VITEK 2 system. A. baumannii

SUBMITTER: Liu S 

PROVIDER: S-EPMC7462923 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature