Serial circulating tumour DNA analysis for locally advanced rectal cancer treated with preoperative therapy: prediction of pathological response and postoperative recurrence.

ABSTRACT:

Background

The "watch-and-wait" approach is a common treatment option amongst patients with locally advanced rectal cancer (LARC). However, the diagnostic sensitivity of clinical modalities, such as colonoscopy and magnetic resonance imaging to determine pathological response, is not high. We analysed the clinical utility of circulating tumour DNA (ctDNA) of patients with LARC to predict response to preoperative therapy and postoperative recurrence.

Methods

A serial ctDNA analysis of 222 plasma samples from 85 patients with LARC was performed using amplicon-based deep sequencing on a cell-free DNA panel covering 14 genes with over 240 hotspots.

Results

ctDNA was detected in 57.6% and 22.3% of samples at baseline and after preoperative treatment, respectively, which wa

SUBMITTER: Murahashi S 

PROVIDER: S-EPMC7462982 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature