Aquatic suspended particulate matter as source of eDNA for fish metabarcoding.

ABSTRACT: The use of environmental DNA (eDNA) for monitoring aquatic macrofauna allows the non-invasive species determination and measurement of their DNA abundance and typically involves the analysis of eDNA captured from water samples. In this proof-of-concept study, we focused on the novel use of eDNA extracted from archived suspended particulate matter (SPM) for identifying fish species using metabarcoding, which benefits from the prospect of retrospective monitoring and also analysis of fish communities through time. We used archived SPM samples of the German Environmental Specimen Bank (ESB), which were collected using sedimentation traps from different riverine points in Germany. Environmental DNA was extracted from nine SPM samples differing in location, organic content, and porosity (among

SUBMITTER: Diaz C 

PROVIDER: S-EPMC7463230 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature