Normal Patterns of Histone H3K27 Methylation Require the Histone Variant H2A.Z in Neurospora crassa.

ABSTRACT: Neurospora crassa contains a minimal Polycomb repression system, which provides rich opportunities to explore Polycomb-mediated repression across eukaryotes and enables genetic studies that can be difficult in plant and animal systems. Polycomb Repressive Complex 2 is a multi-subunit complex that deposits mono-, di-, and trimethyl groups on lysine 27 of histone H3, and trimethyl H3K27 is a molecular marker of transcriptionally repressed facultative heterochromatin. In mouse embryonic stem cells and multiple plant species, H2A.Z has been found to be colocalized with H3K27 methylation. H2A.Z is required for normal H3K27 methylation in these experimental systems, though the regulatory mechanisms are not well understood. We report here that Neurospora crassa mutants lacking H2A.Z

SUBMITTER: Courtney AJ 

PROVIDER: S-EPMC7463285 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature