L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans.

ABSTRACT: Ascorbate (AsA) is required as a cofactor and is widely distributed in plants and animals. Recently, it has been suggested that the nematode Caenorhabditis elegans also synthesizes AsA. However, its biosynthetic pathway is still unknown. To further understand AsA biosynthesis in C. elegans, we analyzed the incorporation of the ¹³C atom into AsA using gas chromatography-mass spectrometry (GC-MS) in worms fed with D-Glc (1-¹³C)-labeled Escherichia coli. GC-MS analysis revealed that AsA biosynthesis in C. elegans, similarly to that in mammalian systems, involves carbon skeleton rearrangement. The addition of L-gulono-1,4-lactone, an AsA precursor in the mammalian pathway, significantly increased AsA level in C. elegans, whereas the addi

SUBMITTER: Yabuta Y 

PROVIDER: S-EPMC7463950 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature