A Numerical Investigation of Enhancing Microfluidic Heterogeneous Immunoassay on Bipolar Electrodes Driven by Induced-Charge Electroosmosis in Rotating Electric Fields.

ABSTRACT: A unique approach is proposed to boost on-chip immuno-sensors, for instance, immunoassays, wherein an antibody immobilized on the walls of a microfluidic channel binds specifically to an antigen suspended freely within a working fluid. The performance of these sensors can be limited in both susceptibility and response speed by the slow diffusive mass transfer of the analyte to the binding surface. Under appropriate conditions, the binding reaction of these heterogeneous immuno-assays may be enhanced by electroconvective stirring driven by external AC electric fields to accelerate the translating motion of antigens towards immobilized antibodies. To be specific, the phenomenon of induced-charge electroosmosis in a rotating electric field (ROT-ICEO) is fully utilized to stir analyte in the v

SUBMITTER: Ge Z 

PROVIDER: S-EPMC7463963 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature