High-Density SNP-Based Association Mapping of Seed Traits in Fenugreek Reveals Homology with Clover.

ABSTRACT: Fenugreek as a self-pollinated plant is ideal for genome-wide association mapping where traits can be marked by their association with natural mutations. However, fenugreek is poorly investigated at the genomic level due to the lack of information regarding its genome. To fill this gap, we genotyped a collection of 112 genotypes with 153,881 SNPs using double digest restriction site-associated DNA sequencing. We used 38,142 polymorphic SNPs to prove the suitability of the population for association mapping. One significant SNP was associated with both seed length and seed width, and another SNP was associated with seed color. Due to the lack of a comprehensive genetic map, it is neither possible to align the newly developed markers to chromosomes nor to predict the underlying genes. Theref

SUBMITTER: Abd El-Wahab MMH 

PROVIDER: S-EPMC7464718 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature