The Potential of HTS Approaches for Accurate Genotyping in Grapevine (Vitis vinifera L.).

ABSTRACT: The main challenge associated with genotyping based on conventional length polymorphisms is the cross-laboratory standardization of allele sizes. This step requires the inclusion of standards and manual sizing to avoid false results. Capillary electrophoresis (CE) approaches limit the information to the length polymorphism and do not allow the determination of a complete marker sequence. As an alternative, high-throughput sequencing (HTS) offers complete information regarding marker sequences and their flanking regions. In this work, we investigated the suitability of a semi-quantitative sequencing approach for microsatellite genotyping using Illumina paired-end technology. Twelve microsatellite loci that are well established for grapevine CE typing were analysed on 96 grapevine samples fr

SUBMITTER: Kunej U 

PROVIDER: S-EPMC7464945 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature