EMS-Induced Mutagenesis of Clostridium carboxidivorans for Increased Atmospheric CO₂ Reduction Efficiency and Solvent Production.

ABSTRACT: Clostridium carboxidivorans (P7) is one of the most important solvent-producing bacteria capable of fermenting syngas (CO, CO₂, and H₂) to produce chemical commodities when grown as an autotroph. This study aimed to develop ethyl methanesulfonate (EMS)-induced P7 mutants that were capable of growing in the presence of CO₂ as a unique source of carbon with increased solvent formation and atmospheric CO₂ reduction to limit global warming. Phenotypic analysis including growth and end product characterization of the P7 wild type (WT) demonstrated that this strain grew better at 25 °C than 37 °C when CO₂ served as the only source of carbon. In the current study, 55 mutagenized P7-EMS mutants were developed by using 100 mM and

SUBMITTER: Lakhssassi N 

PROVIDER: S-EPMC7464951 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature