Comparative Study of Organoids from Patient-Derived Normal and Tumor Colon and Rectal Tissue.

ABSTRACT: Colon and rectal tumors, often referred to as colorectal cancer, show different gene expression patterns in studies that analyze whole tissue biopsies containing a mix of tumor and non-tumor cells. To better characterize colon and rectal tumors, we investigated the gene expression profile of organoids generated from endoscopic biopsies of rectal tumors and adjacent normal colon and rectum mucosa from therapy-naive rectal cancer patients. We also studied the effect of vitamin D on these organoid types. Gene profiling was performed by RNA-sequencing. Organoids from a normal colon and rectum had a shared gene expression profile that profoundly differed from that of rectal tumor organoids. We identified a group of genes of the biosynthetic machinery as rectal tumor organoid-specific, including

SUBMITTER: Costales-Carrera A 

PROVIDER: S-EPMC7465167 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature