Evaluation of Extraction Methods for Clinical Metagenomic Assay.

ABSTRACT: (1) Background: Clinical metagenomics is a promising approach that helps to identify etiological agents in cases of unknown infections. For the efficient detection of an unknown pathogen, the extraction method must be carefully selected for the maximum recovery of nucleic acid from different microorganisms. The aim of this study was to evaluate different extraction methods that have the ability to isolate nucleic acids from different types of pathogens with good quality and quantity for efficient use in clinical metagenomic identification. (2) Methods: A mock sample spiked with five different pathogens was used for the comparative evaluation of different commercial extraction kits. Extracted samples were subjected to library preparation and run on MiSeq. The selected extraction method base

SUBMITTER: Farraj SA 

PROVIDER: S-EPMC7465710 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature