Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation.

ABSTRACT: Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Müller glia in the ERMs from

SUBMITTER: Vishwakarma S 

PROVIDER: S-EPMC7465764 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature