Assembling Reads Improves Taxonomic Classification of Species.

ABSTRACT: Most current approach to metagenomic classification employ short next generation sequencing (NGS) reads that are present in metagenomic samples to identify unique genomic regions. NGS reads, however, might not be long enough to differentiate similar genomes. This suggests a potential for using longer reads to improve classification performance. Presently, longer reads tend to have a higher rate of sequencing errors. Thus, given the pros and cons, it remains unclear which types of reads is better for metagenomic classification. We compared two taxonomic classification protocols: a traditional assembly-free protocol and a novel assembly-based protocol. The novel assembly-based protocol consists of assembling short-reads into longer reads, which will be subsequently classified by a traditiona

SUBMITTER: Tran Q 

PROVIDER: S-EPMC7465921 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature