A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation.

ABSTRACT: A novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in ~10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during the

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7466103 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature