Development and Validation of HPLC-DAD Method for Simultaneous Determination of Seven Food Additives and Caffeine in Powdered Drinks.

ABSTRACT: The usage of food additives must respect the general legislation in force in the country and requires a reliable analytical method for surveillance. This research aimed to develop a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous determination of seven food additives and caffeine in powdered drinks. Three factors likely to affect the chromatographic separation, namely, mobile phase composition at the beginning (x1, 0-10% of the amount of methanol in the phosphate buffer) and the end (x2, 60-100% of the amount of methanol in the phosphate buffer) of the gradient program and pH (x3, 3-7), were evaluated with the aid of a Box-Behnken Design (BBD). Subsequently, multi-response optimizations for chromatographic resolutions (Rs) and analys

SUBMITTER: Imanulkhan 

PROVIDER: S-EPMC7466259 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature