AKT2 drives cancer progression and is negatively modulated by miR-124 in human lung adenocarcinoma.

ABSTRACT:

Background

AKT2 is highly expressed in many human cancers, including non-small cell lung cancer (NSCLC). Accumulating evidence has also revealed that AKT2 can promote NSCLC cell proliferation and metastasis. However, the involved mechanism remains unclear. Herein, our study mainly explored the function of AKT2 during cancer progression and uncovered a new post-transcriptional mechanism of AKT2 expression in lung adenocarcinoma (LUAD).

Methods

Quantitative real-time (qRT-PCR), western blot and immunohistochemistry (IHC) assays were performed to detect the expression of AKT2 and other proteins. Cell counting kit-8 (CCK-8), colony formation and EdU assays were performed to assess cell proliferation. Flow cytometry analysis was used to detect changes in the cell cycle and apopto

SUBMITTER: Liu T 

PROVIDER: S-EPMC7466426 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature