Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions.

ABSTRACT: Isothermal titration calorimetry (ITC) is a powerful and widely used method to measure the energetics of macromolecular interactions by recording a thermogram of differential heating power during a titration. However, traditional ITC analysis is limited by stochastic thermogram noise and by the limited information content of a single titration experiment. Here we present a protocol for bias-free thermogram integration based on automated shape analysis of the injection peaks, followed by combination of isotherms from different calorimetric titration experiments into a global analysis, statistical analysis of binding parameters and graphical presentation of the results. This is performed using the integrated public-domain software packages NITPIC, SEDPHAT and GUSSI. The recently developed lo

SUBMITTER: Brautigam CA 

PROVIDER: S-EPMC7466939 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature