Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.

ABSTRACT: At the oocyte-to-embryo transition the highly differentiated oocyte arrested in meiosis becomes a totipotent embryo capable of embryogenesis. Oocyte maturation (release of the prophase I primary arrest) and egg activation (release from the secondary meiotic arrest and the trigger for the oocyte-to-embryo transition) serve as prerequisites for this transition, both events being controlled posttranscriptionally. Recently, we obtained a comprehensive list of proteins whose levels are developmentally regulated during these events via a high-throughput quantitative proteomic analysis of Drosophila melanogaster oocyte maturation and egg activation. We conducted a targeted screen for potential novel regulators of the oocyte-to-embryo transition, selecting 53 candidates from these proteins.

SUBMITTER: Aviles-Pagan EE 

PROVIDER: S-EPMC7466974 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature