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A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.

ABSTRACT: The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

SUBMITTER: Kim DK

PROVIDER: S-EPMC7467003 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.

Kim Dae-Kyum DK Knapp Jennifer J JJ Kuang Da D Chawla Aditya A Cassonnet Patricia P Lee Hunsang H Sheykhkarimli Dayag D Samavarchi-Tehrani Payman P Abdouni Hala H Rayhan Ashyad A Li Roujia R Pogoutse Oxana O Coyaud Étienne É van der Werf Sylvie S Demeret Caroline C Gingras Anne-Claude AC Taipale Mikko M Raught Brian B Jacob Yves Y Roth Frederick P FP

G3 (Bethesda, Md.) 20200902 9

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block i ...[more]

PMID: 32763951

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