Electron-event representation data enable efficient cryoEM file storage with full preservation of spatial and temporal resolution.

ABSTRACT: Direct detector device (DDD) cameras have revolutionized electron cryomicroscopy (cryoEM) with their high detective quantum efficiency (DQE) and output of movie data. A high ratio of camera frame rate (frames per second) to camera exposure rate (electrons per pixel per second) allows electron counting, which further improves the DQE and enables the recording of super-resolution information. Movie output also allows the correction of specimen movement and compensation for radiation damage. However, these movies come at the cost of producing large volumes of data. It is common practice to sum groups of successive camera frames to reduce the final frame rate, and therefore the file size, to one suitable for storage and image processing. This reduction in the temporal resolution of the camera

SUBMITTER: Guo H 

PROVIDER: S-EPMC7467176 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature