MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition.

ABSTRACT: Human adipose-derived mesenchymal stem cells (hADSCs) are an ideal source of seed cells for regenerative applications and tissue engineering. However, long-term in vitro culture of hADSCs reduces their quantity and quality, which lessens their value in research and clinical applications. The molecular mechanisms underlying this biological process are poorly defined. Recently identified microRNAs (miRNAs) have emerged as critical modulators of cellular senescence. In this study, we examined the changes in hADSCs undergoing senescence. Significant miR-483-3p upregulation was noted during in vitro passaging of hADSCs, which correlated with the adipogenic differentiation and cellular senescence. Knockdown of miR-483-3p retarded the adipogenic differentiation potential of hADSCs a

SUBMITTER: Shen J 

PROVIDER: S-EPMC7467354 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature