Harnessing Conformational Plasticity to Generate Designer Enzymes.

ABSTRACT: Recent years have witnessed an explosion of interest in understanding the role of conformational dynamics both in the evolution of new enzymatic activities from existing enzymes and in facilitating the emergence of enzymatic activity de novo on scaffolds that were previously non-catalytic. There are also an increasing number of examples in the literature of targeted engineering of conformational dynamics being successfully used to alter enzyme selectivity and activity. Despite the obvious importance of conformational dynamics to both enzyme function and evolvability, many (although not all) computational design approaches still focus either on pure sequence-based approaches or on using structures with limited flexibility to guide the design. However, there exist a wide variety of co

SUBMITTER: Crean RM 

PROVIDER: S-EPMC7467679 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature