PhenoChip: A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae.

ABSTRACT: Photosynthetic microorganisms are key players in aquatic ecosystems with strong potential for bioenergy production, yet their systematic selection at the single-cell level for improved productivity or stress resilience ("phenotyping") has remained largely inaccessible. To facilitate the phenotyping of microalgae and cyanobacteria, we developed "PhenoChip," a platform for the multiparametric photophysiological characterization and selection of unicellular phenotypes under user-controlled physicochemical conditions. We used PhenoChip to expose single cells of the coral symbiont Symbiodinium to thermal and chemical treatments and monitor single-cell photophysiology via chlorophyll fluorometry. This revealed strain-specific thermal sensitivity thresholds and distinct pH optima for photosynthet

SUBMITTER: Behrendt L 

PROVIDER: S-EPMC7467707 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature