Ultrafast Vibrational Energy Transfer between Protein and Cofactor in a Flavoenzyme.

ABSTRACT: Protein motions and enzyme catalysis are often linked. It is hypothesized that ultrafast vibrations (femtosecond-picosecond) enhance the rate of hydride transfer catalyzed by members of the old yellow enzyme (OYE) family of ene-reductases. Here, we use time-resolved infrared (TRIR) spectroscopy in combination with stable "heavy" isotopic labeling (²H, ¹³C, ¹⁵N) of protein and/or cofactor to probe the vibrational energy transfer (VET) between pentaerythritol tetranitrate reductase (a member of the OYE family) and its noncovalently bound flavin mononucleotide (FMN) cofactor. We show that when the FMN cofactor is photoexcited with visible light, vibrational energy is transferred from the flavin to the surrounding protein environment on the picosecond timescale

SUBMITTER: Hardman SJO 

PROVIDER: S-EPMC7467709 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature