Ectopic expression of GhSAMDC₁ improved plant vegetative growth and early flowering through conversion of spermidine to spermine in tobacco.

ABSTRACT: Polyamines play essential roles in plant development and various stress responses. In this study, one of the cotton S-adenosylmethionine decarboxylase (SAMDC) genes, GhSAMDC₁, was constructed in the pGWB17 vector and overexpressed in tobacco. Leaf area and plant height increased 25.9-36.6% and 15.0-27.0%, respectively, compared to the wild type, and flowering time was advanced by 5 days in transgenic tobacco lines. Polyamine and gene expression analyses demonstrated that a decrease in spermidine and an increase in total polyamines and spermine might be regulated by NtSPDS₄ and NtSPMS in transgenic plants. Furthermore, exogenous spermidine, spermine and spermidine synthesis inhibitor dicyclohexylamine were used for complementary tests, which resulted in small leaves an

SUBMITTER: Zhu H 

PROVIDER: S-EPMC7468128 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature