VEGF-A splice variants bind VEGFRs with differential affinities.

ABSTRACT: Vascular endothelial growth factor A (VEGF-A) and its binding to VEGFRs is an important angiogenesis regulator, especially the earliest-known isoform, VEGF-A_165a. Yet several additional splice variants play prominent roles in regulating angiogenesis in health and in vascular disease, including VEGF-A₁₂₁ and an anti-angiogenic variant, VEGF-A_165b. Few studies have attempted to distinguish these forms from their angiogenic counterparts, experimentally. Previous studies of VEGF-A:VEGFR binding have measured binding kinetics for VEGFA₁₆₅ and VEGF-A₁₂₁, but binding kinetics of the other two pro- and all anti-angiogenic splice variants are not known. We measured the binding kinetics for VEGF-A₁₆₅, -A_165b, and -A₁₂₁

SUBMITTER: Mamer SB 

PROVIDER: S-EPMC7468149 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature